PKM anticorps (Biotin)

N° du produit ABIN1607677

N° du produit (Fournisseur): 200-1678-0100

L’anticorps Chèvre Polyclonal anti-PKM a été validé pour ELISA et WB. Il convient pour détecter PKM dans des échantillons de Lapin.

Aperçu rapide pour PKM anticorps (Biotin) (ABIN1607677)

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PKM est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Détail du produit anti-PKM anticorps (Biotin)

N° du produit (Fournisseur): 200-1678-0100

Fournisseur: Rockland

Fonction: Anti-Pyruvate Kinase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Attributs du produit: Synonyms: goat anti-Pyruvate Kinase Antibody, biotin Conjugated goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Purification: Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Pyruvate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Pyruvate Kinase Biotin Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.

Western Blot Dilution: 1:200 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur PKM

Antigène: PKM (Pyruvate Kinase M1/2 (PKM))

Autre désignation: PKM

Sujet: Background: Pyruvate Kinase is an enzyme that is key in the glycolytic process. Pyruvate Kinase's specific activity is to catalyze the transfer of a phosphate group from phosphoenolpyruvate to ADP, yielding one molecule of pyruvate and one molecule of ATP. A isoenzyme of Pyruvate Kinase, known as the M2, is a key area of study in tumorigenesis, as the M2 isoenzyme seems to elevate aerobic glycolysis in tumor cells, leading to tumor proliferation. Conflicting evidence as to the importance of this isoenzyme in tumor growth establishes this as a large area of exploration and study. Anti-Pyruvate Kinase Antibody is ideal for investigators in Cancer, Cell Biology, and Signal Transduction research.

ID gène: 100008676

NCBI Accession: NP_001182574

UniProt: P11974

Pathways: L'effet Warburg