ADA anticorps (Biotin)

N° du produit ABIN1607721

N° du produit (Fournisseur): 200-406-140-0100

Cet anticorps Lapin Polyclonal détecte spécifiquement ADA dans WB, ELISA et IP. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour ADA anticorps (Biotin) (ABIN1607721)

Antigène: ADA (Adenosine Deaminase (ADA))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADA est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-ADA anticorps (Biotin)

N° du produit (Fournisseur): 200-406-140-0100

Fournisseur: Rockland

Fonction: Adenosine Deaminase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].

Attributs du produit: Synonyms: rabbit anti-Adenosine Deaminase Antibody biotin Conjugation, biotin conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Adenosine Deaminase [Calf Spleen]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Adenosine Deaminase Biotin Antibody has been tested by western blot. This product is suitable to be assayed against 1.0 μg of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15,000 to 1:64,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur ADA

Antigène: ADA (Adenosine Deaminase (ADA))

Autre désignation: ADA

Sujet: Background: Adenosine Deaminase catalyzes the deamination of adenosine to inosine, thus destabilizing double-stranded RNA. Anti-Adenosine Deaminase is a member of the adenosine and AMP deaminases family. This antibody is a factor in purine metabolism and adenosine homeostasis, as well as positively regulating T-cell coactivation through the binding of DPP4. The antibody interacts with DPP4 in the extracellular domain, thus regulating lymphocyte-epithelial cell adhesion. It moderates extracellular adenosine signaling. Enzyme deficiency in Adenosine Deaminase has been associated with severe combined immunodeficiency disease, whereas an overabundance may result in congenital hemolytic anemia. Anti-Adenosine Deaminase is ideal for investigators involved in Enzyme, DNA and RNA research.

ID gène: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process