ADA anticorps (Biotin)
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- Antigène Voir toutes ADA Anticorps
- ADA (Adenosine Deaminase (ADA))
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Reactivité
- Boeuf (Vache)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp ADA est conjugé à/à la Biotin
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Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Réactivité croisée (Details)
- Cross reactivity against Adenosine Deaminase from other sources may occur but has not been specifically determined.
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].
- niveau d'endotoxine
- Low Endotoxin : No
- Immunogène
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Adenosine Deaminase [Calf Spleen]
Immunogen Type: Native Protein - Isotype
- IgG
- Top Product
- Discover our top product ADA Anticorps primaire
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- Indications d'application
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This product has been assayed against 1.0 µg of Adenosine Deaminase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15.000 to 1:64.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 10 mg/mL
- Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - Conseil sur la manipulation
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Date de péremption
- Expiration date is one (1) year from date of opening.
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- Antigène
- ADA (Adenosine Deaminase (ADA))
- Autre désignation
- Adenosine Deaminase (ADA Produits)
- Synonymes
- anticorps ADA-like, anticorps xada, anticorps ADA, anticorps CG11994, anticorps Dmel\\CG11994, anticorps DrosADA, anticorps dADA, anticorps zgc:92028, anticorps adenosine deaminase, anticorps adenosine deaminase S homeolog, anticorps Adenosine deaminase, anticorps ADA, anticorps Ada, anticorps ada.S, anticorps ada
- Sujet
- Adenosine Deaminase catalyzes the deamination of adenosine to inosine, thus destabilizing double-stranded RNA. Anti-Adenosine Deaminase is a member of the adenosine and AMP deaminases family. This antibody is a factor in purine metabolism and adenosine homeostasis, as well as positively regulating T-cell coactivation through the binding of DPP4. The antibody interacts with DPP4 in the extracellular domain, thus regulating lymphocyte-epithelial cell adhesion. It moderates extracellular adenosine signaling. Enzyme deficiency in Adenosine Deaminase has been associated with severe combined immunodeficiency disease, whereas an overabundance may result in congenital hemolytic anemia. Anti-Adenosine Deaminase is ideal for investigators involved in Enzyme, DNA and RNA research. Synonyms: Adenosine deaminase EC=3.5.4.4 Adenosine aminohydrolase
- ID gène
- 280712
- UniProt
- P56658
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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