Alkaline Phosphatase anticorps (Biotin)

N° du produit ABIN1607761

N° du produit (Fournisseur): 200-4634-0100

L’anticorps Lapin Polyclonal anti-Alkaline Phosphatase a été validé pour WB et ELISA. Il convient pour détecter Alkaline Phosphatase dans des échantillons de E. coli.

Aperçu rapide pour Alkaline Phosphatase anticorps (Biotin) (ABIN1607761)

Antigène: Alkaline Phosphatase (ALP)

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Alkaline Phosphatase est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-Alkaline Phosphatase anticorps (Biotin)

N° du produit (Fournisseur): 200-4634-0100

Fournisseur: Rockland

Fonction: Alkaline Phosphatase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.

Attributs du produit: Synonyms: rabbit anti-Alkaline Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody

Purification: Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Alkaline Phosphatase [E.coli]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase

Sujet: Background: At a high pH , Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research.

ID gène: 945041

NCBI Accession: NP_414917

UniProt: P00634