Glucose Oxidase anticorps (Biotin)

N° du produit ABIN1607767

N° du produit (Fournisseur): 200-4637-0100

L’anticorps Lapin Polyclonal anti-Glucose Oxidase a été validé pour ELISA et WB. Il convient pour détecter Glucose Oxidase dans des échantillons de Aspergillus niger.

Aperçu rapide pour Glucose Oxidase anticorps (Biotin) (ABIN1607767)

Antigène: Glucose Oxidase (GOD)

Reactivité: Aspergillus niger

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Glucose Oxidase est conjugé à/à la Biotin

Application: ELISA, Western Blotting (WB)

Détail du produit anti-Glucose Oxidase anticorps (Biotin)

N° du produit (Fournisseur): 200-4637-0100

Fournisseur: Rockland

Fonction: Glucose Oxidase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Glucose Oxidase [A.

Attributs du produit: Synonyms: rabbit anti-Glucose Oxidase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Anti-GLUCOSE OXIDASE Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Glucose Oxidase [A.niger]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-GLUCOSE OXIDASE Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 μg of Glucose Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Glucose Oxidase

Antigène: Glucose Oxidase (GOD)

Autre désignation: Glucose Oxidase

Sujet: Background: Glucose Oxidase antibody detect the glucose oxidase protein. The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-δ-lactone. In cells, it aids in breaking the sugar down into its metabolites. Glucose oxidase is widely used for the determination of free glucose in body fluids (diagnostics), in vegetal raw material, and in the food industry. GOx is a dimeric protein, the 3D structure of which has been elucidated. The active site where glucose binds is in a deep pocket. The enzyme, like many proteins that act outside of cells, is covered with carbohydrate chains. At pH 7, glucose exists in solution in cyclic hemiacetal form as 63.6 % β-D-glucopyranose and 36.4 % α-D-glucopyranose, the proportion of linear and furanose form being negligible. Anti-Glucose Oxidase antibody is ideal for investigators involved in glucose energy metabolism research.

ID gène: 2683500

UniProt: P13006