Urease anticorps (Biotin)

N° du produit ABIN1607815

N° du produit (Fournisseur): 200-4682-0100

L’anticorps Lapin Polyclonal anti-Urease a été validé pour WB, ELISA et IP. Il convient pour détecter Urease dans des échantillons de Haricots-sabres.

Aperçu rapide pour Urease anticorps (Biotin) (ABIN1607815)

Antigène: Urease (URE)

Reactivité: Haricots-sabres

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Urease est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Urease anticorps (Biotin)

N° du produit (Fournisseur): 200-4682-0100

Fournisseur: Rockland

Fonction: Urease Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].

Attributs du produit: Synonyms: rabbit anti-Urease Antibody biotin Conjugation, biotin Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody

Purification: Anti-Urease antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Urease [Jack Bean]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Urease biotin conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Urease

Antigène: Urease (URE)

Autre désignation: Urease

Sujet: Background: Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.

UniProt: P07374