RNASE1 anticorps (Biotin)

N° du produit ABIN1607821

Détail du produit anti-RNASE1 anticorps (Biotin)

Antigène: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RNASE1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Réactivité croisée (Details): Cross reactivity against Ribonuclease A from other sources may occur but have not been specifically determined.

Pureté: Anti-Ribonuclease A antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas].

niveau d'endotoxine: Low Endotoxin : No

Immunogène: Ribonuclease A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application: Anti-Ribonuclease A antibody has been assayed against 1.0 µg of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:13.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Conseil sur la manipulation: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Date de péremption: Expiration date is one (1) year from date of opening.

Détails sur RNASE1

Antigène: RNASE1 (Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))

Autre désignation: Ribonuclease A (RNASE1 Produits)

Synonymes: anticorps RNASE1, anticorps RNS1, anticorps ATRNS1, anticorps RIBONUCLEASE 1, anticorps T17M13.16, anticorps T17M13_16, anticorps ribonuclease 1, anticorps RIB1, anticorps AI574248, anticorps Rib-1, anticorps Rib1, anticorps SRN, anticorps ribonuclease A family member 1, pancreatic, anticorps ribonuclease pancreatic-like, anticorps ribonuclease A family member k6, anticorps Ribonuclease 1, anticorps ribonuclease 1, anticorps ribonuclease, RNase A family, 1 (pancreatic), anticorps ribonuclease pancreatic, anticorps RNASE1, anticorps LOC475395, anticorps RNASE6, anticorps Rnase1, anticorps RNS1, anticorps LOC101113761

Sujet: Anti-Ribonuclease A recognizes ribonuclease, an endoribonuclease that catalyzes the cleavage of the phosphodiester bond between 5'-ribose of a nucleotide and the phosphate group of a 3'-ribose of a pyrimidine nucleotide. Able to survive acidic and near boiling conditions, RNase A is highly stable. RNase A and its homologs have been demonstrated to have cytotoxic and cytostatic effects. Synonyms: Pancreatic ribonuclease antibody, Rib 1 antibody, Rib1 antibody, Ribonuclease a family 1 antibody, Ribonuclease pancreatic antibody, Ribonuclease RNase A family 1 antibody, Ribonuclease RNase A family 1 pancreatic antibody

ID gène: 282340

UniProt: P61823