LPO anticorps (Biotin)

N° du produit ABIN1607841

N° du produit (Fournisseur): 200-606-146-0100

Cet anticorps Mouton Polyclonal détecte spécifiquement LPO dans WB, IP, ELISA et DB. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour LPO anticorps (Biotin) (ABIN1607841)

Antigène: LPO (Lactoperoxidase (LPO))

Reactivité: Boeuf (Vache)

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp LPO est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA, Dot Blot (DB)

Détail du produit anti-LPO anticorps (Biotin)

N° du produit (Fournisseur): 200-606-146-0100

Fournisseur: Rockland

Fonction: Anti-Lactoperoxidase Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Lactoperoxidase [Bovine Milk].

Attributs du produit: Synonyms: sheep anti-Lactoperoxidase biotin conjugated Antibody, biotin conjugated sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody

Purification: Anti-Lactoperoxidase (Bovine Milk) (Sheep) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Lactoperoxidase [Bovine Milk]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Lactoperoxidase Biotin Conjugated Antibody has been tested by Dot blot and western blot and is suitable to be assayed against 1.0 μg of Lactoperoxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for Anti-Lactoperoxidase.

Western Blot Dilution: 1:50 - 1:500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:500 - 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur LPO

Antigène: LPO (Lactoperoxidase (LPO))

Autre désignation: Lactoperoxidase

Sujet: Background: Lactoperoxidase antibody recognizes the lactoperoxidase protein. Lactoperoxidase catalyzes the oxidation of a number of inorganic and organic substrates by hydrogen peroxide. Lactoperoxidase plays an important role in killing bacteria in milk. Lactoperoxidase conjugated to Biotin is used to detect the specific target of the lactoperoxidase protein. Lactoperoxidase conjugated to Biotin is suitable for researchers in immunology and biochemistry.

ID gène: 280844

NCBI Accession: NP_776358

UniProt: P80025