Albumin anticorps (HRP)
Aperçu rapide pour Albumin anticorps (HRP) (ABIN1607852)
Antigène
Voir toutes Albumin (ALB) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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N° du produit (Fournisseur)
- 201-4333-0100
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Fournisseur
- Rockland
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Fonction
- Bovine Serum Albumin Antibody Peroxidase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum].
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Attributs du produit
- Synonyms: rabbit anti-bovine albumin peroxidase conjugation, rabbit anti-bovine albumin HRP conjugation, rabbit anti-BSA HRP conjugated antibody, rabbit anti-bovine serum albumin peroxidase conjugated antibody, ALB antibody, Albumin antibody, HRP conjugated Anti-Bovine serum albumin antibody
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Purification
- Anti-BOVINE ALBUMIN (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Stérilité
- Sterile filtered
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Immunogène
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Immunogen: Albumin (Bovine Serum)
Immunogen Type: Native Protein
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry Dilution: 1:250 - 1:1,000
Application Note: Anti-BOVINE ALBUMIN Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for dot blot, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. It is also used as a nutrient in cell and microbial culture. Specific conditions should be optimized by user.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Agent conservateur
- Gentamicin sulfate
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Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C
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Stockage commentaire
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- Albumin (ALB)
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Autre désignation
- ALB
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Sujet
- Background: Bovine serum albumin (also known as BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard. BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels. This protein does not affect other enzymes that do not need it for stabilization. BSA is also commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. BSA is used because of its stability to increase signal in assays, its lack of effect in many biochemical reactions, and its low cost, since large quantities of it can be readily purified from bovine blood, a byproduct of the cattle industry. Anti-Bovine albumin antibody is ideal for investigators involved in serum protein component research.
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ID gène
- 280717
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UniProt
- P02769
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Pathways
- Lipid Metabolism
Antigène
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