GABBR2 anticorps (AA 861-912)

N° du produit ABIN1686619

Cet anticorps Souris Monoclonal détecte spécifiquement GABBR2 dans WB, IHC, ICC et IF. Il présente une réactivité envers Rat.

Aperçu rapide pour GABBR2 anticorps (AA 861-912) (ABIN1686619)

Antigène: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GABBR2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S81-2

Détail du produit anti-GABBR2 anticorps

Épitope: AA 861-912

Specificité: Detects ~105 kDa. No cross-reactivity against GABA(B)R1.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 861-912 of rat GABA(B)R2

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1686619 was sufficient for detection of GABA(B)R2 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur GABBR2

Antigène: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Autre désignation: GABA B Receptor 2

Sujet: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

ID gène: 83633

UniProt: O88871

Pathways: cAMP Metabolic Process