Perforin 1 anticorps (AA 22-100)

N° du produit ABIN1713880

Détail du produit anti-Perforin 1 anticorps

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Épitope: AA 22-100

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Perforin 1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Réactivité croisée: Souris, Rat

Homologie: Human,Horse

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human Perforin

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur Perforin 1

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Autre désignation: Perforin (PRF1 Produits)

Synonymes: anticorps FLH2, anticorps HPLH2, anticorps P1, anticorps PFN1, anticorps PFP, anticorps PRF1, anticorps Pfn, anticorps Pfp, anticorps Prf-1, anticorps Cyta, anticorps RATCYTA, anticorps LOC443187, anticorps perforin, anticorps prf1, anticorps cytolysin, anticorps perforin-1, anticorps perforin-1-like, anticorps perforin 1, anticorps perforin 1 (pore forming protein), anticorps perforin, anticorps perforin 1 L homeolog, anticorps PRF1, anticorps Prf1, anticorps LOC443187, anticorps prf1, anticorps prf1.L

Sujet:

Synonyms: Cytolysin, FLH2, HPLH2, Lymphocyte pore forming protein, Lymphocyte pore-forming protein, MGC65093, P1, PERF_HUMAN, Perforin 1, Perforin 1 precursor, Perforin 1 preforming protein, Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF, Pore forming protein, PRF 1, PRF1, SHGC-10760.

Background: The major defense of the body against virus-infected and tumorigenic cells is cytotoxic T lymphocyte (CTL)-mediated cytotoxicity, which also plays a role in autoimmune diseases and transplant rejection. During CTL-mediated cytotoxicity, CTL granules containing perforin are exocytosed. Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells by forming transmembrane channels in target cell membranes. Perforin is primarily expressed in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, but has also been observed in an astrocyte population of the human brain. It has been shown that abrogation of perforin function by Ca2+-complexing agents leads to decreased levels of necrosis, demonstrating that both necrosis and apoptosis contribute to CTL-mediated cytotoxicity. Perforin activity has been shown to be induced by IL-2, IL-3, IL-4, IL-6 and to a lesser degree, TNF and IFN-_.

ID gène: 5551

Pathways: Apoptose, Caspase Cascade in Apoptosis