AGO2 anticorps

N° du produit ABIN1720882

Cet anticorps Lapin Polyclonal détecte spécifiquement AGO2 dans WB. Il présente une réactivité envers Arabidopsis thaliana.

Aperçu rapide pour AGO2 anticorps (ABIN1720882)

Antigène: AGO2 (Argonaute 2 (AGO2))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-AGO2 anticorps

Specificité: TAIR: At1g48410

Réactivité croisée (Details): Not reactive in: Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays

Homologie: Capsella rubella, Solanum tuberosum

Purification: serum

Immunogène: KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Information d'application

Indications d'application: 1: 250 - 1: 500 (WB)

Commentaires:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Détails sur AGO2

Antigène: AGO2 (Argonaute 2 (AGO2))

Autre désignation: AGO2

Sujet: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Poids moléculaire: expected: 116.4 kDa, appearent: 130 kDa

UniProt: Q9SHF3

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization