AGO2 anticorps

N° du produit ABIN1720882

Détail du produit anti-AGO2 anticorps

Antigène: AGO2 (Argonaute 2 (AGO2))

Reactivité: Arabidopsis thaliana

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO2 est non-conjugé

Application: Western Blotting (WB)

Specificité: TAIR: At1g48410

Réactivité croisée (Details): Not reactive in: Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays

Homologie: Capsella rubella, Solanum tuberosum

Purification: serum

Immunogène: KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280

Information d'application

Indications d'application: 1: 250 - 1: 500 (WB)

Commentaires:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Détails sur AGO2

Antigène: AGO2 (Argonaute 2 (AGO2))

Autre désignation: AGO2 (AGO2 Produits)

Synonymes: anticorps EIF2C2, anticorps T19E23.7, anticorps T19E23_7, anticorps argonaute 2, anticorps Q10, anticorps Eif2c2, anticorps Argonaute2, anticorps eif2c1, anticorps eif2c2, anticorps 1110029L17Rik, anticorps 2310051F07Rik, anticorps AI225898, anticorps AL022874, anticorps AW546247, anticorps ENSMUSG00000072493, anticorps Gerp95, anticorps Gm10365, anticorps mKIAA4215, anticorps AGO2, anticorps AG02, anticorps AGO 2, anticorps Ago-2, anticorps Ago2, anticorps CG13452, anticorps CG7439, anticorps Dm Ago2, anticorps Dmel\\CG7439, anticorps ago, anticorps ago-2, anticorps ago2, anticorps dAGO2, anticorps dAgo2, anticorps eIF2C2, anticorps argonaute 2, RISC catalytic component, anticorps Argonaute family protein, anticorps argonaute 2, RISC catalytic component L homeolog, anticorps argonaute RISC catalytic subunit 2, anticorps argonaute RISC catalytic component 2, anticorps Argonaute 2, anticorps AGO2, anticorps Ago2, anticorps ago2.L, anticorps ago2

Sujet: AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

Poids moléculaire: expected: 116.4 kDa, appearent: 130 kDa

UniProt: Q9SHF3

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization