SHANK3 anticorps (AA 538-626) (PE)

N° du produit ABIN1741267

Cet anticorps Souris Monoclonal détecte spécifiquement SHANK3 dans WB, IHC, ICC et IF. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour SHANK3 anticorps (AA 538-626) (PE) (ABIN1741267)

Antigène: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SHANK3 est conjugé à/à la PE

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: S367-51

Détail du produit anti-SHANK3 anticorps (PE)

Épitope: AA 538-626

Specificité: Detects ~190 kDa. Cross-reacts with SHANK1. Does not cross-react with SHANK2.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 538-626 (SH3 domain) of rat SHANK3. Mouse: 100% identity (89/89 amino acids identical). Human: 97% identity (87/89 amino acids identical). ~70% identity with SHANK1 and SHANK2.

Isotype: IgG2a

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1741267 was sufficient for detection of SHANK1/SHANK3 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur SHANK3

Antigène: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Autre désignation: SHANK3

Sujet: SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development.

ID gène: 59312

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity