HSF1 anticorps (AA 425-439) (Atto 488)

N° du produit ABIN1741559

L’anticorps Rat Monoclonal anti-HSF1 a été validé pour WB, ELISA, IHC, IP, IF, GS et ICC. Il convient pour détecter HSF1 dans des échantillons de Souris.

Aperçu rapide pour HSF1 anticorps (AA 425-439) (Atto 488) (ABIN1741559)

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSF1 est conjugé à/à la Atto 488

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Gel Shift (GS), Immunocytochemistry (ICC)

Clone: 4B4

Détail du produit anti-HSF1 anticorps (Atto 488)

Épitope: AA 425-439

Specificité: Detects ~85 kDa (unstressed cell lysates) and ~95 kDa (heat shocked cell lysates).

Réactivité croisée: Boeuf (Vache), Cobaye, Hamster, Humain, Singe, Souris, Lapin, Rat

Purification: Protein G Purified

Immunogène: Purified recombinant mouse HSF1 protein, epitope mapping to amino acids 425-439

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1741559 was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Rabbit anti-rat IgG: AP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: HSF1

Sujet: HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

ID gène: 15499

NCBI Accession: NP_032322

UniProt: P38532