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NSF anticorps (AA 733-744)

L’anticorps Lapin Polyclonal anti-NSF a été validé pour WB, ELISA, IP, IHC et ICC. Il convient pour détecter NSF dans des échantillons de Humain, Rat, Souris et Hamster. Il y a 5+ publications disponibles.
N° du produit ABIN1742270

Aperçu rapide pour NSF anticorps (AA 733-744) (ABIN1742270)

Antigène

Voir toutes NSF Anticorps
NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivité

  • 50
  • 31
  • 31
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Humain, Rat, Souris, Hamster

Hôte

  • 61
  • 6
  • 1
Lapin

Clonalité

  • 64
  • 4
Polyclonal

Conjugué

  • 35
  • 5
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp NSF est non-conjugé

Application

  • 55
  • 25
  • 13
  • 13
  • 9
  • 9
  • 8
  • 7
  • 6
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Épitope

    • 15
    • 9
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 733-744

    Fonction

    Rabbit anti-NSF antiserum

    Specificité

    Specific for NSF.

    Réactivité croisée (Details)

    Reacts with: human (P46459), rat (Q9QUL6), mouse (P46460), hamster. Other species not tested yet.

    Purification

    antiserum

    Immunogène

    Synthetic peptide corresponding to AA 733 to 744 from rat NSF (UniProt Id: Q9QUL6)
  • Indications d'application

    WB:1 : 1000 , IP:yes ICC:1 : 100 up to 1 : 1000, IHC:1 : 200, IHC-P:not tested yet ELISA:yes

    Commentaires

    ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742269 as capture antibody (protocol for sandwich-ELISA).

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 200 μLH2O, then aliquot and store at -20 °C until use.

    Conseil sur la manipulation

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    Stock

    -20 °C,4 °C

    Stockage commentaire

    Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
  • de Paola, Bello, Michaut: "Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes." dans: PLoS ONE, Vol. 10, Issue 8, pp. e0135679, (2015) (PubMed).

    Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." dans: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

    Tian, Wu, Unzicker, Lu, Cai, Li, Schirra, Matti, Stevens, Deng, Rettig, Sheng: "The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 45, pp. 10546-55, (2005) (PubMed).

    Tailleux, Neyrolles, Honoré-Bouakline, Perret, Sanchez, Abastado, Lagrange, Gluckman, Rosenzwajg, Herrmann: "Constrained intracellular survival of Mycobacterium tuberculosis in human dendritic cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1939-48, (2003) (PubMed).

    Michaut, Tomes, De Blas, Yunes, Mayorga: "Calcium-triggered acrosomal exocytosis in human spermatozoa requires the coordinated activation of Rab3A and N-ethylmaleimide-sensitive factor." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 18, pp. 9996-10001, (2000) (PubMed).

  • Antigène

    NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

    Autre désignation

    NSF

    Sujet

    Synonyms: SNARE associated, N-ethylmaleimide-sensitive factor, NEM-sensitive fusion protein, SKD2

    Antigen Information: NSF is a trimeric ATPase required for membrane fusion during vesicular transport

    UniProt

    Q9QUL6
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