GAD65 anticorps (N-Term)

N° du produit ABIN1742417

L’anticorps Souris Monoclonal anti-GAD65 a été validé pour WB, IHC, ELISA, IP, ICC et IHC (p). Il convient pour détecter GAD65 dans des échantillons de Humain, Rat et Souris. Il y a 3+ publications disponibles.

Aperçu rapide pour GAD65 anticorps (N-Term) (ABIN1742417)

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAD65 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 26H1D1

Détail du produit anti-GAD65 anticorps

Épitope: N-Term

Fonction: Mouse anti-GAD2 IgG

Specificité: Specific for GAD 2 / GAD 65

Réactivité croisée (Details): Reacts with: rat (Q05683), mouse (P48320), human (Q05329). Other species not tested yet.

Purification: purified IgG

Immunogène: Recombinant protein corresponding to the amino terminus of mouse GAD2 (UniProt Id: P48320) Epitop AA 3 to 17 from mouse GAD2 (UniProt Id: P48320)

Isotype: IgG3

Information d'application

Indications d'application: WB:1 : 1000 , IP:yes ICC:1 : 500, IHC:1 : 200 up to 1 : 500, IHC-P:1 : 100 up to 1 : 1000, ELISA:yes

Commentaires:

WB: This antibody is less sensitive than the rabbit antibody. ELISA: Suitable as capture antibody for sandwich-ELISA with ABIN1742419 as detector antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C,-80 °C,4 °C

Stockage commentaire: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Références pour anticorps anti-GAD65 (ABIN1742417)

Pan, Marrs, Ryan: "Vesicular glutamate transporter 1 orchestrates recruitment of other synaptic vesicle cargo proteins during synaptic vesicle recycling." dans: The Journal of biological chemistry, Vol. 290, Issue 37, pp. 22593-601, (2015) (PubMed).

Liu, Bickford, Held, Nyitrai, Südhof, Kaeser: "The active zone protein family ELKS supports Ca2+ influx at nerve terminals of inhibitory hippocampal neurons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 37, pp. 12289-303, (2014) (PubMed).

Coley, Ruffin, Moss, Hopfer, Boron: "Immunocytochemical identification of electroneutral Na⁺-coupled HCO₃⁻ transporters in freshly dissociated mouse medullary raphé neurons." dans: Neuroscience, Vol. 246, pp. 451-67, (2013) (PubMed).

Détails sur GAD65

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Autre désignation: GAD2

Sujet:

Synonyms: Glutamic acid decarboxylase, GAD65, Glutamic acid decarboxylase 2, Glutamic acid decarboxylase 65

Antigen Information: GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter

UniProt: P48320