GAD65 anticorps (N-Term)

N° du produit ABIN1742419

Cet anticorps Lapin Polyclonal détecte spécifiquement GAD65 dans WB, IHC, ELISA, IP, ICC et IHC (p). Il présente une réactivité envers Rat et Souris et a été mentionné dans 2+ publications.

Aperçu rapide pour GAD65 anticorps (N-Term) (ABIN1742419)

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivité: Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAD65 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GAD65 anticorps

Épitope: N-Term

Fonction: Rabbit anti-GAD2 antibody

Specificité: Specific for GAD 2 / GAD 65.

Réactivité croisée (Details): Reacts with: rat (P18088), mouse (P48318). Other species not tested yet.

Purification: affinity purified

Immunogène: Recombinant protein corresponding to the amino terminus of mouse GAD2 (UniProt Id: P48320)

Information d'application

Indications d'application: WB:1 : 1000 , IP:yes ICC:1 : 2000, IHC:1 : 1000, IHC-P:1 : 1000, ELISA:yes

Commentaires:

ICC/IHC: The crude antiserum is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742417 as capture antibody (protocol for sandwich-ELISA).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: -20 °C,-80 °C,4 °C

Stockage commentaire: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

Références pour anticorps anti-GAD65 (ABIN1742419)

Du, Chazalon, Bestaven, Leste-Lasserre, Baufreton, Cazalets, Cho, Garret: "Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." dans: Neuroscience, Vol. 329, pp. 363-79, (2016) (PubMed).

Saliba, Gu, Yan, Moss: "Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition." dans: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32544-50, (2009) (PubMed).

Détails sur GAD65

Antigène: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Autre désignation: GAD2

Sujet:

Synonyms: Glutamic acid decarboxylase, GAD65, Glutamic acid decarboxylase 2, Glutamic acid decarboxylase 65

Antigen Information: GAD1 and 2 are involved in the synthesis of GABA, the major inhibitory neurotransmitter

UniProt: P48320