TUBB3 anticorps (AA 443-450)

N° du produit ABIN1742553

Cet anticorps anti-TUBB3 est un anticorps Lapin Polyclonal détectant TUBB3 dans WB, IHC, IP et ICC. Adapté pour Humain, Souris et Rat. Validé indépendamment pour une utilisation dans Western Blotting. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour TUBB3 anticorps (AA 443-450) (ABIN1742553)

Antigène: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TUBB3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-TUBB3 anticorps

Épitope: AA 443-450

Specificité: Specific for beta3-tubulin.

Immunogène: Synthetic peptide (aa 443-450 of mouse beta3-tubulin) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine.

Information d'application

Indications d'application: WB: 1:1000 up to 1:10000, ICC: 1:1000 up to 1:5000, IHC: 1:200 up to 1:500, IHC-P: 1:2000 up to 1:4000,

Restrictions: For Research Use only

Independent Validation (WB)

Validation #029822 (Western Blotting)

by: Kinexus Bioinformatics Corporation

No.: #029822

Date: 28.10.2014

Numéro du lot: 302302/3

Application validée: Western Blotting

Contrôle positif: Mouse brain

Contrôle négative: Mouse heart, HeLa cells, A431 cells

Conclusion: A band was observed in the positive controls at the expected size (~50 kDa), which is lower in the negative controls.

Validation Images

Figure 1: Western Blot for Tubulin, Beta, 3 (TUBB3). Grey arrowhead indicates the expected molecular weight of ~50 kDa.

Protocole

Anticorps primaire:

• Antigen: Tubulin, Beta, 3 (TUBB3) (AA 443-450)
• Catalog number: ABIN1742553
• Lot number: 302302/3
• Dilution: 1:1000

Anticorps secondaire:

• Antibody: Donkey anti-Rabbit IgG Antibody (HRP)
• Lot number: F0613
• Dilution: 1:10,000

Full Protocol:

• 1. Cell/tissue total protein lysates were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 minutes prior to loading.
• 2. 15 μg of boiled lysate were loaded and resolved on a 12% SDS-polyacrylamide gel.
• 3. The Precision Plus Protein™ All Blue Prestained Standards from BioRad (161-0373) were used as molecular mass markers.
• 4. Proteins were transferred onto nitrocellulose membrane by tank transfer and protein transfer was confirmed with Ponceau S staining.
• 5. The immunoblot membrane was blocked in 2.5% skim milk and 1.5% BSA solution in TTBS at room temperature for 60 minutes.
• 6. The membrane was washed in TTBS twice for 5 minutes each.
• 7. The membrane was immersed with the protein side up in the antibody solution in TBS and incubated overnight at 4°C with gentle agitation.
• 8. The membrane was rinsed twice with TTBS.
• 9. The membrane was washed in TTBS twice for 5 minutes each.
• 10. The membrane was washed in TTBS once for 15 minutes.
• 11. The membrane was incubated in the HRP-conjugated secondary antibody solution in TBS for 60 minutes at room temperature with gentle agitation.
• 12. The membrane was rinsed twice with TTBS.
• 13. The membrane was washed in TTBS twice for 5 minutes each.
• 14. The membrane was washed in TTBS once for 15 minutes.
• 15. Signals were detected by chemiluminescence (ECL). The blot was scanned for 320 seconds.
• 16. The membrane was rinsed three times with TTBS.
• 17. Repeated Steps 4-15 with the loading control antibody and its matching secondary antibody. The blot was scanned for 160 seconds.

Notes: - No experimental challenges noted.

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 200µl H2O.

Agent conservateur: Azide and BSA free

Conseil sur la manipulation: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Stock: 4 °C/-20 °C

Stockage commentaire: Reconstitute and aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized.

Références pour anticorps anti-TUBB3 (ABIN1742553)

Kaczor, Rakus, Mozrzymas: "Neuron-astrocyte interaction enhance GABAergic synaptic transmission in a manner dependent on key metabolic enzymes." dans: Frontiers in cellular neuroscience, Vol. 9, pp. 120, (2015) (PubMed).

Détails sur TUBB3

Antigène: TUBB3 (Tubulin, beta 3 (TUBB3))

Autre désignation: beta3-Tubulin (TUBB3)

Sujet: Synonyms: TuJ1

Pathways: Dynamique des Microtubules, M Phase