Cet anticorps anti-Profilin est un anticorps Souris Monoclonal détectant Profilin dans WB, ICC et IHC. Adapté pour Humain, Boeuf (Vache), Cobaye et Lapin. Ce Primary Antibody a été cité dans 6+ publications.
PFN
Reactivité: Plantes
ELISA, WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
WB: 1:500 up to 1:5000, IHC-P: 1:1000,
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 100µl H2O to get a 1mg/ml solution in PBS.
Buffer
PBS.
Agent conservateur
Azide and BSA free
Conseil sur la manipulation
Do not freeze! Antibodies should be stored at +4°C when still lyophilized.
Stock
-20 °C
Stockage commentaire
Reconstitute and aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized.
Lorente, Syriani, Morales: "Actin filaments at the leading edge of cancer cells are characterized by a high mobile fraction and turnover regulation by profilin I." dans: PLoS ONE, Vol. 9, Issue 1, pp. e85817, (2014) (PubMed).
Giesemann, Schwarz, Nawrotzki, Berhörster, Rothkegel, Schlüter, Schrader, Schindelin, Mendel, Kirsch, Jockusch: "Complex formation between the postsynaptic scaffolding protein gephyrin, profilin, and Mena: a possible link to the microfilament system." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 23, pp. 8330-9, (2003) (PubMed).
Giesemann, Rathke-Hartlieb, Rothkegel, Bartsch, Buchmeier, Jockusch, Jockusch: "A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems." dans: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37908-14, (2000) (PubMed).
Janke, Schlüter, Jandrig, Theile, Kölble, Arnold, Grinstein, Schwartz, Estevéz-Schwarz, Schlag, Jockusch, Scherneck: "Suppression of tumorigenicity in breast cancer cells by the microfilament protein profilin 1." dans: The Journal of experimental medicine, Vol. 191, Issue 10, pp. 1675-86, (2000) (PubMed).
Schlüter, Schleicher, Jockusch: "Effects of single amino acid substitutions in the actin-binding site on the biological activity of bovine profilin I." dans: Journal of cell science, Vol. 111 ( Pt 22), pp. 3261-73, (1999) (PubMed).
Wiedemann, Giehl, Almo, Fedorov, Girvin, Steinberger, Rüdiger, Ortner, Sippl, Dolecek, Kraft, Jockusch, Valenta: "Molecular and structural analysis of a continuous birch profilin epitope defined by a monoclonal antibody." dans: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 29915-21, (1997) (PubMed).