Cytokeratin 18 anticorps (Internal Region)

N° du produit ABIN1781972

L’anticorps Chèvre Polyclonal anti-Cytokeratin 18 a été validé pour WB, IHC et ELISA. Il convient pour détecter Cytokeratin 18 dans des échantillons de Humain et Porc.

Aperçu rapide pour Cytokeratin 18 anticorps (Internal Region) (ABIN1781972)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-Cytokeratin 18 anticorps

Épitope: Internal Region

Fonction: keratin 18

Séquence: EELDKYWSQQ IE

Specificité: Reported variants represent identical protein: NP_000215.1, NP_954657.1.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Breast, Placenta and Small Intestine. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1 kDa according to NP_000215.1) and approx 45 kDa in lysates of Pig Liver (calculated MW of 47.4 kDa according to XP_005652636.2). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: KRT18

Sujet: KRT18, keratin 18, CYK18, K18, CK-18, cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, cytokeratin 18, cytokeratin-18, keratin, type I cytoskeletal 18, keratin-18

Poids moléculaire: Expected molecular weight: 48 kDa

ID gène: 3875, 16668, 294853

NCBI Accession: NP_000215

Pathways: Apoptose, Caspase Cascade in Apoptosis