HSF1 anticorps (N-Term)

N° du produit ABIN180344

L’anticorps Rat Monoclonal anti-HSF1 a été validé pour WB, IP et IF. Il convient pour détecter HSF1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour HSF1 anticorps (N-Term) (ABIN180344)

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Humain, Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSF1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 4B4

Détail du produit anti-HSF1 anticorps

Épitope: AA 1-503, N-Term

Specificité: Highly specific for HSF1. Detects an ~85 kDa protein in unstressed cell lysates, and an ~95 kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS-PAGE immunoblots.

Réactivité croisée (Details): Species reactivity (expected):Human, Rat, Bovine, Guinea Pig, Hamster, Monkey, RabbitSpecies reactivity (tested):Mouse

Attributs du produit: Synonyms: HSTF1, HSF-1, Heat shock transcription factor 1

Purification: Immunoglobulin fraction purified by ammonium sulphate precipitation

Immunogène: Recombinant mouse HSF1 protein (aa1-503).

Isotype: IgG1

Information d'application

Indications d'application: Immunofluorescence. Immunoprecipitation (Native only): use Protein G and Ab at 2g/mgprotein lysate). Western Blotting: Ab 1-2g/mL for 2hrs at RT. Recommended positivecontrol: LS174T or MAD109 Cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2-8 °C for up to one month or in aliquots at -20 °C for longer. Avoid repeated freezethaw cycles!
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: Heat Shock Factor 1 / HSF1

Sujet: HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents, binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

ID gène: 3297

UniProt: Q00613