Nerve Growth Factor anticorps

N° du produit ABIN181153

Cet anticorps Souris Monoclonal détecte spécifiquement Nerve Growth Factor dans WB, Func et EIA. Il présente une réactivité envers Humain.

Aperçu rapide pour Nerve Growth Factor anticorps (ABIN181153)

Antigène: Nerve Growth Factor (NGF)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nerve Growth Factor est non-conjugé

Application: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Détail du produit anti-Nerve Growth Factor anticorps

Specificité: This antibody detects NGF-beta.

Attributs du produit: Synonyms: Beta-NGF, NGFB, Beta-nerve growth factor

Purification: Affinity Chromatography on Protein G

Immunogène: Highly pure (>98%) recombinant Human beta-NGF

Isotype: IgG2b

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman beta-NGF (10.0 ng/mL), a concentration of 2.7-4.0 μg/mL of this antibody isrequired. ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect at least 200 pg/mL of recombinant Human Beta-NGF when used withBiotin anti-Human beta-NGF antibody (cat. PP1057B) as the detection antibody at aconcentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can beused at a concentration of 4.0-8.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human beta-NGF is 4.0-8.0 ng/lane, underreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Agent conservateur: Without preservative

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Nerve Growth Factor

Antigène: Nerve Growth Factor (NGF)

Autre désignation: NGF

Sujet: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

ID gène: 4803

UniProt: P01138

Pathways: Regulation of Cell Size