CD40 Ligand anticorps

N° du produit ABIN181580

Cet anticorps Chèvre Polyclonal détecte spécifiquement CD40 Ligand dans WB, IHC (p) et EIA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD40 Ligand anticorps (ABIN181580)

Antigène: CD40 Ligand (CD40LG)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CD40 Ligand est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-CD40 Ligand anticorps

Specificité: This antibody detects Human sCD40 Ligand / CD154.

Purification: Immunoaffinity Chromatography employing immobilized Human sCD40 Ligand matrix

Immunogène: Highly pure (> 98%) E.coli derived recombinant Human sCD40L

Information d'application

Indications d'application: ELISA: Indirect: To detect hsCD40-Ligand by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatiblesecondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinanthsCD40-Ligand. Sandwich: To detect hsCD40-Ligand by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5 - 2.0 μg/mL is required. In conjunction with BiotinylatedAnti-Human sCD40-Ligand as a detection antibody, it allows the detection of at least0.2 - 0.4 ng/well of recombinant hsCD40-Ligand. Western Blot: To detect hsCD40-Ligand by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hsCD40-Ligand is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for at least two weeks or (in aliquots) at -20 °C for longer.

Détails sur CD40 Ligand

Antigène: CD40 Ligand (CD40LG)

Autre désignation: CD154 / CD40L

Sujet: CD40 ligand (CD40L) is a 33 kDa type II membrane glycoprotein expressed mainly on the cell surface of activated T lymphocytes, but also exists as a soluble form extracellularly. CD40L is the ligand for CD40, a member of the TNF superfamily, which is expressed on the cell surface of B cells, macrophages/monocytes, dendritic cells, vascular endothelial cells, and epithelial cells. CD40L plays an important role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with B cells expressing CD40, and activation of CD4+ T cells. Mutation within the CD40L gene is linked to hyper IgM syndrome, an X linked immunodeficiency disease that is characterized by elevated level of serum IgM and decreased level of other isotypes.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Poids moléculaire: approx. 150 kDa

ID gène: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints