FGF17 anticorps
Aperçu rapide pour FGF17 anticorps (ABIN181584)
Antigène
Voir toutes FGF17 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects Fibroblast Growth Factor 17.
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Purification
- Immunoaffinity chromatography
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Immunogène
- Highly pure (> 98 %) recombinant human FGF-17
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Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-17 (1.5 ng/mL), a concentration of 0.020 - 0.035 μg/mL of this antibody is required. ELISA: Indirect: To detect hFGF-17 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Sandwich: To detect hFGF-17 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. This In conjunction with BiotinylatedAnti-Human FGF-17 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-17. Western Blot: To detect hFGF-17 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-17 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
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Buffer
- PBS, pH 7.2
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
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Date de péremption
- 6 months
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- FGF17 (Fibroblast Growth Factor 17 (FGF17))
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Autre désignation
- FGF17
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Sujet
- FGF17 belongs to the heparin-binding growth factors family. It may be a signaling molecule in the induction and patterning of the embryonic brain and is preferentially expressed in the embryonic brain. There are two named isoforms.Synonyms: FGF-17, Fibroblast growth factor 17
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ID gène
- 8822
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NCBI Accession
- NP_003858
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UniProt
- O60258
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Pathways
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Antigène
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