APOE anticorps (Isoform E3) (Biotin)
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- Antigène Voir toutes APOE Anticorps
- APOE (Apolipoprotein E (APOE))
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Épitope
- Isoform E3
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp APOE est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificité
- This antibody detects ApoE3. Cross reacts with Human ApoE2 and Human ApoE4 in both Sandwich ELISA and Western Blot.
- Purification
- Affinity Chromatography.
- Immunogène
- E.Coli derived recombinant Human ApoE3.
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- Discover our top product APOE Anticorps primaire
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- Indications d'application
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Direct ELISA: To detect Human ApoE3 by Direct ELISA (using 100 μL/well antibody solution)a concentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human ApoE3. Sandwich ELISA: To detect Human ApoE3 by Sandwich ELISA (using 100 μL/well) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This Biotinylated antibody, inconjunction with Affinity purified anti-Human ApoE3 (ABIN181667 or ABIN181668) as acapture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant HumanApoE3. Western blot: To detect Human ApoE3 this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant Human ApoE3 is 1.5-3.0 ng/lane, under either reducing or non-reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
- Buffer
- PBS, pH 7.2 without preservatives.
- Agent conservateur
- Without preservative
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- Date de péremption
- 6 months
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- Antigène
- APOE (Apolipoprotein E (APOE))
- Autre désignation
- Apolipoprotein E (Apo E) (APOE Produits)
- Synonymes
- anticorps ad2, anticorps apoprotein, anticorps im:7036787, anticorps wu:fb69a05, anticorps zgc:110064, anticorps apoe, anticorps AI255918, anticorps AD2, anticorps LDLCQ5, anticorps LPG, anticorps APOEA, anticorps Apo-E, anticorps apolipoprotein E, anticorps apolipoprotein Ea, anticorps apoe, anticorps apoea, anticorps Apoe, anticorps APOE
- Sujet
- Apolipoprotein E3 mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo E receptor (chylomicron remnant) of hepatic tissues.Synonyms: Apo-E, ApoE
- ID gène
- 348
- NCBI Accession
- NP_000032
- UniProt
- P02649
- Pathways
- Regulation of Cell Size, Lipid Metabolism
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