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MIA2 anticorps (Biotin)

L’anticorps Lapin Polyclonal anti-MIA2 a été validé pour WB et EIA. Il convient pour détecter MIA2 dans des échantillons de Humain.
N° du produit ABIN181709

Aperçu rapide pour MIA2 anticorps (Biotin) (ABIN181709)

Antigène

Voir toutes MIA2 Anticorps
MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

Reactivité

  • 41
  • 4
  • 4
  • 4
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
Humain

Hôte

  • 40
  • 1
Lapin

Clonalité

  • 40
  • 1
Polyclonal

Conjugué

  • 22
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp MIA2 est conjugé à/à la Biotin

Application

  • 25
  • 21
  • 14
  • 6
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Purification

    Affinity chromatography

    Immunogène

    Highly pure (> 98 %) recombinant human MIA-2
  • Indications d'application

    ELISA: Direct: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIA-2. Sandwich: To detect hMIA-2 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-HumanMIA-2 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hMIA-2. Western blot: To detect hMIA-2 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant hMIA-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/mL.

    Buffer

    PBS, pH 7.2

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

    Date de péremption

    6 months
  • Antigène

    MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))

    Autre désignation

    MIA2

    Sujet

    MIA2 (Melanoma inhibitory activity 2) is a secreted cytokine and a member of the MIA/OTOR family. It is predominantly expressed in hepatocytes and may play a role in the pathophysiology of liver disease. Elevated levels of MIA2 may represent a clinically useful marker for diagnosis of hepatic disease activity and severity. MIA2 may act as a tumor suppressor in hepatocellular carcinoma.Synonyms: Melanoma inhibitory activity protein 2

    ID gène

    9606

    UniProt

    Q96PC5
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