This antibody recognizes the Murine scavenger receptor class A (SRA), type I and II, also known as CD204. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. We recommend the use of SM029LE for this purpose. Recent research shows that clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 Mouse strain.
Purification
Affinity Chromatography on Protein G
Immunogène
Raw 264 cell line. Remarks: Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.
MSR1
Reactivité: Humain, Souris, Rat
IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on frozen sections. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions.Synonyms: MSR1, Macrophage acetylated LDL receptor I and II, Macrophage scavenger receptor types I and II, SCARA1, Scavenger receptor class A member 1