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alpha Tubulin anticorps (Subunit alpha) (HRP)

L’anticorps Rat Monoclonal anti-alpha Tubulin a été validé pour WB, ELISA, IHC et IHC (fro). Il convient pour détecter alpha Tubulin dans des échantillons de Humain, Souris, Rat, Saccharomyces cerevisiae, Arabidopsis, Drosophila melanogaster, Xenopus laevis et C. elegans.
N° du produit ABIN1820258

Aperçu rapide pour alpha Tubulin anticorps (Subunit alpha) (HRP) (ABIN1820258)

Antigène

Voir toutes alpha Tubulin (TUBA1) Anticorps
alpha Tubulin (TUBA1)

Reactivité

  • 106
  • 88
  • 78
  • 22
  • 17
  • 15
  • 13
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  • 2
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  • 1
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Humain, Souris, Rat, Saccharomyces cerevisiae, Arabidopsis, Drosophila melanogaster, Xenopus laevis, C. elegans

Hôte

  • 55
  • 54
  • 11
  • 2
  • 2
  • 1
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Rat

Clonalité

  • 74
  • 50
  • 2
Monoclonal

Conjugué

  • 81
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  • 5
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  • 3
  • 3
  • 3
  • 2
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  • 1
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Cet anticorp alpha Tubulin est conjugé à/à la HRP

Application

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  • 6
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  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

YL1-2
  • Épitope

    • 12
    • 9
    • 8
    • 6
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    • 2
    • 2
    • 2
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    • 1
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    Subunit alpha

    Specificité

    Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

    Aucune reactivité croisée

    Nephrotoma

    Purification

    Protein G purified

    Immunogène

    Yeast tubulin.

    Type of Immunogen: Purified protein

    Isotype

    IgG2a
  • Indications d'application

    Approved: ELISA, IHC, IHC-Fr, WB (1:100 - 1:1000)

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Commentaires

    Target Species of Antibody: Yeast

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, 0.01 % Thimerosal, HRP stabilizer

    Agent conservateur

    Thimerosal (Merthiolate)

    Précaution d'utilisation

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Do not freeze.

    Stock

    4 °C

    Stockage commentaire

    Store at 4°C. Do not freeze.
  • Antigène

    alpha Tubulin (TUBA1)

    Autre désignation

    Alpha Tubulin

    Pathways

    Dynamique des Microtubules
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