His Tag anticorps (iFluor 488)

N° du produit ABIN1842280

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans . Il présente une réactivité envers .

Aperçu rapide pour His Tag anticorps (iFluor 488) (ABIN1842280)

Antigène: His Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est conjugé à/à la iFluor 488

Application: Veuillez nous consulter SVP

Détail du produit anti-His Tag anticorps (iFluor 488)

Marque: THE™

Séquence: HHHHHH

Specificité: THETM His Tag Antibody [iFluor 488], mAb, Mouse recognizes N-terminal, internal and C-terminal His-tagged proteins.

Purification: Protein A affinity column

Immunogène: A synthetic peptide HHHHHH coupled to KLH

Isotype: IgG1 kappa

Information d'application

Indications d'application: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/mL
Flow cytometry: 1-4 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

Concentration: 0.5 mg/mL

Buffer: lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: The lyophilized product remains stable up to 2 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.

Détails sur His Tag

Antigène: His Tag

Classe de substances: Tag

Sujet: The His tag is a short amino acid sequence consisting of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.THETM His Tag Antibody [iFluor 488], mAb, Mouse is THETM His Tag Antibody (A00186) conjugated with iFluor 488 under optimal conditions with an F/P ratio of 4-8. It is suitable for detecting the expression level of His-tagged proteins. iFluor 488 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 488.