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DYKDDDDK Tag anticorps (iFluor 555)

Cet anticorps anti-DYKDDDDK Tag Monoclonal Souris conjugué à iFluor 555 (ABIN1842288) détecte spécifiquement DYKDDDDK Tag.
N° du produit ABIN1842288
290,38 €
Plus frais de livraison 40,00 € et TVA
100 μg
Destination: France
Envoi sous 8 à 12 jours ouvrables

Aperçu rapide pour DYKDDDDK Tag anticorps (iFluor 555) (ABIN1842288)

Antigène

DYKDDDDK Tag

Reactivité

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Hôte

  • 91
  • 86
  • 10
  • 9
  • 6
Souris

Clonalité

  • 103
  • 99
Monoclonal

Conjugué

  • 60
  • 18
  • 13
  • 9
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp DYKDDDDK Tag est conjugé à/à la iFluor 555

Application

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  • Séquence

    DYKDDDDK

    Specificité

    THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse recognizes N-terminal, internal and C-terminal Flag-tagged proteins.

    Purification

    Protein A affinity column

    Immunogène

    A synthetic peptide DYKDDDDK coupled to KLH

    Isotype

    IgG2b kappa
  • Indications d'application

    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    Immunofluorescent staining: 1-4 µg/mL
    Other applications: user-optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.

    Concentration

    0.5 mg/mL

    Buffer

    lyophilized with PBS, pH 7.4, containing 10 mg/mL BSA and 0.02 % sodium azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    The lyophilized product remains stable up to 2 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below.
  • Antigène

    DYKDDDDK Tag

    Classe de substances

    Tag

    Sujet

    The DYKDDDK tag is a polypeptide with the amino acid sequence DYKDDDDK that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of the protein to facilitate detection and purification. An anti-DYKDDDK tag antibody is a useful tool for the analysis of Flag-tagged proteins.THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse is THETM DYKDDDDK Tag Antibody (A00187) conjugated with iFluor 555 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of DYKDDDDK-tagged proteins. iFluor 555 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 555.
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