DDB1 anticorps (C-Term)

N° du produit ABIN184570

Cet anticorps Chèvre Polyclonal détecte spécifiquement DDB1 dans WB, IHC et ELISA. Il présente une réactivité envers Humain et Souris et a été mentionné dans 1 publication.

Aperçu rapide pour DDB1 anticorps (C-Term) (ABIN184570)

Antigène: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DDB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-DDB1 anticorps

Épitope: C-Term

Fonction: DDB1

Séquence: DLIKVVEELT RIH

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DLIKVVEELTRIH, from the C Terminus of the protein sequence according to NP_001914.3.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 140 kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat (calculated MW of 126.9 kDa according to Human NP_001914.3). Recommended concentration: 1-2 μg/mL. Approx 140 kDa band observed in lysates of cell line NIH3T3 and app

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-DDB1 (ABIN184570)

Li, Robert, van Breugel, Strubin, Zheng: "A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery." dans: Nature structural & molecular biology, Vol. 17, Issue 1, pp. 105-11, (2010) (PubMed).

Détails sur DDB1

Antigène: DDB1 (Damage Specific DNA Binding Protein 1 (DDB1))

Autre désignation: DDB1

Sujet: DDB1, damage-specific DNA binding protein 1, 127 kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE

ID gène: 1642, 64470

NCBI Accession: NP_001914

Pathways: Réparation de l'ADN