NCF1 anticorps (C-Term)

N° du produit ABIN184583

L’anticorps Chèvre Polyclonal anti-NCF1 a été validé pour WB, ELISA, IHC, IF et FACS. Il convient pour détecter NCF1 dans des échantillons de Humain, Souris et Porc.

Aperçu rapide pour NCF1 anticorps (C-Term) (ABIN184583)

Antigène: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivité: Humain, Souris, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NCF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-NCF1 anticorps

Épitope: C-Term

Fonction: NCF1 / p47phox

Séquence: SESTKRKLAS AV

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SESTKRKLASAV, from the C Terminus of the protein sequence according to NP_000256.3.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Colon, Neutrophils. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in lysates of cell lines Daudi, U251 and U937 and in Mouse Thymus, and approx, 50 kDa in Pig Spleen lysates (calculated MW of 44.7 kDa according to Human NP_000256.4, 46.0 kDa according to Mouse NP_001272966.1 and 45

Peptide ELISA: antibody detection limit dilution 1:20000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NCF1

Antigène: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Autre désignation: NCF1

Sujet: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2

ID gène: 653361, 17969, 114553

NCBI Accession: NP_000256

Pathways: Signalisation PI3K-Akt