STAP2 anticorps (C-Term)

N° du produit ABIN184610

L’anticorps Chèvre Polyclonal anti-STAP2 a été validé pour WB et ELISA. Il convient pour détecter STAP2 dans des échantillons de Humain. Il y a 4+ publications disponibles.

Aperçu rapide pour STAP2 anticorps (C-Term) (ABIN184610)

Antigène: STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp STAP2 est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-STAP2 anticorps

Épitope: C-Term

Fonction: STAP2 / BKS

Séquence: ELQKKLEKRR ALEH

Specificité: This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-ELQKKLEKRRALEH, from the C Terminus of the protein sequence according to NP_060190.2, NP_001013863.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 48 kDa band observed in Human Heart lysates and 45 kDa band observed in Human Breast Lysates (calculated MW of 49.6 kDa according to NP_060190 and of NP_001013863.1). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-STAP2 (ABIN184610)

Ikeda, Mizushima, Sekine, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells." dans: Cancer science, Vol. 102, Issue 4, pp. 756-61, (2011) (PubMed).

Ikeda, Sekine, Mizushima, Nakasuji, Miyasaka, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells." dans: The Journal of biological chemistry, Vol. 285, Issue 49, pp. 38093-103, (2010) (PubMed).

Ikeda, Miyasaka, Sekine, Mizushima, Muromoto, Nanbo, Yoshimura, Matsuda: "STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation." dans: Biochemical and biophysical research communications, Vol. 384, Issue 1, pp. 71-5, (2009) (PubMed).

Sekine, Ikeda, Tsuji, Yamamoto, Muromoto, Nanbo, Oritani, Yoshimura, Matsuda: "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7966-74, (2009) (PubMed).

Détails sur STAP2

Antigène: STAP2 (Signal Transducing Adaptor Family Member 2 (STAP2))

Autre désignation: STAP2

Sujet: STAP2, BKS, STAP-2, FLJ20234, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein-2, signal transducing adaptor family member 2

ID gène: 55620

NCBI Accession: NP_060190, NP_001013863