RACGAP1 anticorps (C-Term)

N° du produit ABIN184690

Cet anticorps Chèvre Polyclonal détecte spécifiquement RACGAP1 dans WB, IHC, ELISA, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour RACGAP1 anticorps (C-Term) (ABIN184690)

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RACGAP1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-RACGAP1 anticorps

Épitope: C-Term

Fonction: RACGAP1 / MgcRacGAP

Séquence: GRQGNFFASP MLK

Specificité: This antibody is expected to recognize all reported isoforms (NP_037409.2, NP_001306934.1, NP_001306935.1, NP_001306936.1). Reported variants represent identical protein: NP_001306928.1, NP_001119576.1, NP_037409.2, NP_001306931.1, NP_001306929.1, NP_0011

Réactivité croisée: Boeuf (Vache), Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GRQGNFFASPMLK, from the C Terminus of the protein sequence according to NP_037409.2, NP_001119576.1, NP_001119575.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0 kDa according to NP_037409.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room te

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur RACGAP1

Antigène: RACGAP1 (Rac GTPase Activating Protein 1 (RACGAP1))

Autre désignation: RACGAP1

Sujet: RACGAP1, MgcRacGAP, Rac GTPase activating protein 1, ID-GAP, MGCRACGAP, GTPase activating protein, HsCYK-4, HsCYK-4

ID gène: 29127

NCBI Accession: NP_037409, NP_001306934, NP_001306935, NP_001306936

Pathways: Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling of Hepatocyte Growth Factor Receptor