UBE2C anticorps (C-Term)

N° du produit ABIN184805

L’anticorps Chèvre Polyclonal anti-UBE2C a été validé pour WB, ELISA, IF, IP et FACS. Il convient pour détecter UBE2C dans des échantillons de Humain.

Aperçu rapide pour UBE2C anticorps (C-Term) (ABIN184805)

Antigène: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2C est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-UBE2C anticorps

Épitope: C-Term

Fonction: UBE2C / UBCH10

Séquence: QETYSKQVTS QEP

Specificité: This antibody is expected to recognise all reported isoforms. Variants NP_861517.1 and NP_861518.1 encode the same isoform.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1, NP_861515.1, NP_861516.1, NP_861517.1, NP_861518.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7 kDa according to NP_008950.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur UBE2C

Antigène: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Autre désignation: UBE2C

Sujet: UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1

ID gène: 11065

NCBI Accession: NP_008950, NP_861515, NP_861516, NP_861517, NP_861518, NP_001268670

Pathways: Ubiquitin Proteasome Pathway