KAP1 anticorps (C-Term)

N° du produit ABIN184913

L’anticorps Chèvre Polyclonal anti-KAP1 a été validé pour ELISA, IHC, IF et FACS. Il convient pour détecter KAP1 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour KAP1 anticorps (C-Term) (ABIN184913)

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp KAP1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-KAP1 anticorps

Épitope: C-Term

Fonction: KAP1 / TRIM28

Séquence: SSQELSGGPG DGP

Réactivité croisée: Humain, Souris

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-KAP1 (ABIN184913)

Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." dans: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).

Détails sur KAP1

Antigène: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Autre désignation: TRIM28

Sujet: TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein

ID gène: 10155

NCBI Accession: NP_005753

Pathways: Signalisation Hedgehog, Positive Regulation of Response to DNA Damage Stimulus