CHFR anticorps (C-Term)

N° du produit ABIN184918

Cet anticorps Chèvre Polyclonal détecte spécifiquement CHFR dans ELISA et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour CHFR anticorps (C-Term) (ABIN184918)

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CHFR est non-conjugé

Application: ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-CHFR anticorps

Épitope: C-Term

Fonction: CHFR

Séquence: KFNHICEQTR FKN

Specificité: This antibody is expected to recognise all reported isoforms.

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1, NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in human brain lysates at 0.5 μg/mL, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur CHFR

Antigène: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Autre désignation: CHFR

Sujet: CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196

ID gène: 55743, 231600, 288734

NCBI Accession: NP_001154816, NP_001154817, NP_001154818, NP_060693, NP_001154819