DPF1 anticorps (C-Term)

N° du produit ABIN184922

L’anticorps Chèvre Polyclonal anti-DPF1 a été validé pour ELISA et IF. Il convient pour détecter DPF1 dans des échantillons de Humain.

Aperçu rapide pour DPF1 anticorps (C-Term) (ABIN184922)

Antigène: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DPF1 est non-conjugé

Application: ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-DPF1 anticorps

Épitope: C-Term

Fonction: Neuro-d4 / DPF1

Séquence: HLKEKASAYI TLT

Specificité: This antibody is expected to recognize all three reported isoforms (NP_001128627.1, NP_004638.2, NP_001128628.1).

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-HLKEKASAYITLT, from the C Terminus of the protein sequence according to NP_001128627.1, NP_004638.2, NP_001128628.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary experiments showed a band at approx 38-40 kDa in Human Cerebellum and Frontal Cortex lysates after 0.3-1 μg/mL antibody staining (calculated MW of 37.9 kDa according to NP_001128628.1. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei and weaker staining in the cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur DPF1

Antigène: DPF1 (D4, Zinc and Double PHD Fingers Family 1 (DPF1))

Autre désignation: DPF1

Sujet: DPF1, NEUD4, neuro-d4, D4, zinc and double PHD fingers family 1, Neuro-d4 (rat) homolog, D4, zinc and double PHD fingers, family 1, MGC150428, MGC150429, BAF45b, OTTHUMP00000211615, neuro-d4 homolog

ID gène: 8193, 29861, 50545

NCBI Accession: NP_001128627, NP_004638, NP_001128628