PSMF1 anticorps (C-Term)

N° du produit ABIN184933

Cet anticorps Chèvre Polyclonal détecte spécifiquement PSMF1 dans WB, ELISA et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PSMF1 anticorps (C-Term) (ABIN184933)

Antigène: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PSMF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-PSMF1 anticorps

Épitope: C-Term

Fonction: PI31 / PSMF1 (Isoform 1)

Séquence: DHLPPPGYDD MYL

Specificité: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: C-DHLPPPGYDDMYL

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 30 kDa band observed in lysates of cell line HEK293, approx 30-32 kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35 kDa in lysates of cell line LNCaP (calculated MW of 29.8 kDa according to Human NP_006805.2 an

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry:A customer reported weak staining of A431 cells at

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PSMF1

Antigène: PSMF1 (Proteasome (Prosome, Macropain) Inhibitor Subunit 1 (PI31) (PSMF1))

Autre désignation: PSMF1

Sujet: PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1

ID gène: 9491, 228769

NCBI Accession: NP_006805

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA