PARK7/DJ1 anticorps (C-Term)

N° du produit ABIN184951

Cet anticorps Chèvre Polyclonal détecte spécifiquement PARK7/DJ1 dans WB, IHC, ELISA et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PARK7/DJ1 anticorps (C-Term) (ABIN184951)

Antigène: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PARK7/DJ1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-PARK7/DJ1 anticorps

Épitope: C-Term

Fonction: PARK7 / DJ-1

Séquence: AAQVKAPLVL KD

Specificité: Reported variants (NP_001116849.1, NP_009193.2) represent the same protein.

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-AAQVKAPLVLKD, from the C Terminus of the protein sequence according to NP_001116849.1, NP_009193.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Testis. Recommended concentration: 7 μg/mL.

Western Blot: Approx 24 kDa band observed in lysates of cell lines HeLa and Jurkat and of Human Brain (Cerebellum, Hippocampus, Frontal Cortex) and rodent Brain lysates (calculated MW of 19.9 kDa according to NP_001116849.1 and of NP_009193.2). Recommended

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen around the nucleus. Dividing cells appear to show enhanced expression. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur PARK7/DJ1

Antigène: PARK7/DJ1 (PARK7) (Parkinson Protein 7 (PARK7))

Autre désignation: PARK7

Sujet: PARK7, DJ1, DJ-1, Parkinson disease (autosomal recessive, early onset) 7, oncogene DJ1, CTA-215D11.1, FLJ27376, FLJ34360, FLJ92274, OTTHUMP00000001350, OTTHUMP00000001351, Parkinson disease protein 7, protein DJ-1

ID gène: 11315, 57320, 117287

NCBI Accession: NP_009193

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Proton Transport