REV1 anticorps (C-Term)

N° du produit ABIN185012

L’anticorps Chèvre Polyclonal anti-REV1 a été validé pour IHC et ELISA. Il convient pour détecter REV1 dans des échantillons de Humain.

Aperçu rapide pour REV1 anticorps (C-Term) (ABIN185012)

Antigène: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp REV1 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-REV1 anticorps

Épitope: C-Term

Fonction: REV1L

Séquence: VLQQTYGSTL KVT

Specificité: This antibody is expected to recognise both reported isoforms.

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-VLQQTYGSTLKVT, from the C Terminus of the protein sequence according to NP_057400.1, NP_001032961.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6 μg/mL.

Western Blot: No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur REV1

Antigène: REV1 (REV1, Polymerase (DNA Directed) (REV1))

Autre désignation: REV1

Sujet: REV1, REV1L, REV1-like (yeast), REV1, REV1 protein, REV1 (yeast homolog)- like, REV1 homolog (S. cerevisiae), FLJ21523, MGC163283, MGC26225, REV1- like

ID gène: 51455, 56210

NCBI Accession: NP_057400, NP_001032961

Pathways: Réparation de l'ADN, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response