FARP1 anticorps (N-Term)

N° du produit ABIN185104

Détail du produit anti-FARP1 anticorps

Antigène: FARP1 (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FARP1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: CDEP / FARP1

Séquence: GEIEQRPTPG SRL

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence GEIEQRPTPGSRL-C, from the N Terminus of the protein sequence according to NP_005757.1, NP_001001715.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary experiments showed an approx 110-120 kDa band in Human Frontal Cortex lysates (calculated MW of 119 kDa according to NP_ 005757.1).. An additional band was also consistently observed at 40 kDa and was successfully blocked by incuba

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur FARP1

Antigène: FARP1 (FERM, RhoGEF (ARHGEF) and Pleckstrin Domain Protein 1 (Chondrocyte-Derived) (FARP1))

Autre désignation: FARP1 (FARP1 Produits)

Synonymes: anticorps FARP1, anticorps CDEP, anticorps PLEKHC2, anticorps PPP1R75, anticorps AW228844, anticorps BC030329, anticorps Cdep, anticorps FERM, ARH/RhoGEF and pleckstrin domain protein 1, anticorps FERM, RhoGEF (Arhgef) and pleckstrin domain protein 1 (chondrocyte-derived), anticorps FARP1, anticorps Farp1

Sujet: FARP1, FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived), CDEP, chondrocyte-derived ezrin-like protein, RP11-111L24.1, MGC87400, PLEKH2, FERM, RhoGEF, and pleckstrin domain protein 1

ID gène: 10160

NCBI Accession: NP_005757, NP_001001715, NP_001273768