POLL anticorps (C-Term)

N° du produit ABIN185143

Cet anticorps Chèvre Polyclonal détecte spécifiquement POLL dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour POLL anticorps (C-Term) (ABIN185143)

Antigène: POLL (Polymerase (DNA Directed), lambda (POLL))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp POLL est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-POLL anticorps

Épitope: C-Term

Fonction: POLL / POLkappa

Séquence: LGLPYREPAE RDW

Specificité: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32 % in the core) but considering only

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-LGLPYREPAERDW, from the C Terminus of the protein sequence according to NP_037406.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 60 kDa band observed in cell lysates of A549 and approx 60-70 kDa in cell lysates of HepG2 (calculated MW of 63.5 kDa according to Human NP_037406.1). Recommended concentration: 0.5-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur POLL

Antigène: POLL (Polymerase (DNA Directed), lambda (POLL))

Autre désignation: POLL

Sujet: POLL, BETA-N, POL-KAPPA, polymerase (DNA directed), lambda, polymerase (DNA-directed), lambda, DNA polymerase kappa (DNA polymerase beta-N), RP11-529I10.1, BETAN, FLJ46002, POLKAPPA, DNA polymerase beta-2, DNA polymerase kappa (DNA polymerase beta-N), DNA

ID gène: 27343, 56626

NCBI Accession: NP_037406

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response