SH3BP1 anticorps (C-Term)

N° du produit ABIN185183

L’anticorps Chèvre Polyclonal anti-SH3BP1 a été validé pour WB, ELISA, IF, IHC et ICC. Il convient pour détecter SH3BP1 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour SH3BP1 anticorps (C-Term) (ABIN185183)

Antigène: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SH3BP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Classe de qualité: Verified

Détail du produit anti-SH3BP1 anticorps

Épitope: C-Term

Fonction: SH3BP1

Séquence: PQPRPRSLAS ETN

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PQPRPRSLASETN, from the C Terminus of the protein sequence according to NP_061830.3.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Kidney, stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus. Recommended concentration: 0.3 μg/mL.

Western Blot: Approx 70 kDa band observed in Human Lung and Kidney lysates (calculated MW of 75.7 kDa according to NP_061830.3). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunocytochemistry: This antibody has proved to work very well in Caco-2 cells in Elbediwy et al, J.Biol. Chem, 2012 (in press).

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-SH3BP1 (ABIN185183)

Parrini, Sadou-Dubourgnoux, Aoki, Kunida, Biondini, Hatzoglou, Poullet, Formstecher, Yeaman, Matsuda, Rossé, Camonis: "SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility." dans: Molecular cell, Vol. 42, Issue 5, pp. 650-61, (2011) (PubMed).

Détails sur SH3BP1

Antigène: SH3BP1 (SH3-Domain Binding Protein 1 (SH3BP1))

Autre désignation: SH3BP1

Sujet: SH3BP1, SH3-domain binding protein 1, SH3 domain binding protein 1, OTTHUMP00000028981

ID gène: 23616

NCBI Accession: NP_061830