SMUG1 anticorps (N-Term)

N° du produit ABIN185204

Détail du produit anti-SMUG1 anticorps

Antigène: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Épitope: N-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SMUG1 est non-conjugé

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: SMUG1

Séquence: PQAFLLGSIH EPA

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence PQAFLLGSIHEPA-C, from the N Terminus of the protein sequence according to NP_055126.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Preliminary testing showed a band at approx 19 kDa in lysate of cell line HeLa after 0.1 μg/mL antibody staining (calculated MW of 19.6 kDa according to NP_001230718.1),. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U205 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-SMUG1 (ABIN185204)

Ma, Au, Waye: "Over-expression of SUMO-1 induces the up-regulation of heterogeneous nuclear ribonucleoprotein A2/B1 isoform B1 (hnRNP A2/B1 isoform B1) and uracil DNA glycosylase (UDG) in hepG2 cells." dans: Cell biochemistry and function, Vol. 27, Issue 4, pp. 228-37, (2009) (PubMed).

Détails sur SMUG1

Antigène: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Autre désignation: SMUG1 (SMUG1 Produits)

Sujet: SMUG1, single-strand selective monofunctional uracil DNA glycosylase, FDG, HMUDG, UNG3, single-strand-selective monofunctional uracil-DNA glycosylase 1, MGC104370

ID gène: 23583

NCBI Accession: NP_055126

Pathways: Réparation de l'ADN