PPARD anticorps (C-Term)

N° du produit ABIN185282

L’anticorps Chèvre Polyclonal anti-PPARD a été validé pour WB et ELISA. Il convient pour détecter PPARD dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour PPARD anticorps (C-Term) (ABIN185282)

Antigène: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PPARD est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-PPARD anticorps

Épitope: C-Term

Fonction: PPAR delta (Isoform 1)

Séquence: CHPLLQEIYK DMY

Specificité: This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence CHPLLQEIYKDMY, from the C Terminus of the protein sequence according to NP_006229.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 55 kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9 kDa according to NP_006229.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PPARD (ABIN185282)

Minamiyama, Takemura, Kodai, Shinkawa, Tsukioka, Ichikawa, Naito, Yoshikawa, Okada: "Iron restriction improves type 2 diabetes mellitus in Otsuka Long-Evans Tokushima fatty rats." dans: American journal of physiology. Endocrinology and metabolism, Vol. 298, Issue 6, pp. E1140-9, (2010) (PubMed).

Détails sur PPARD

Antigène: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Autre désignation: PPARD

Sujet: PPARD, FAAR, NUC1, NUCI, NR1C2, NUCII, PPARB, MGC3931, PPAR-beta, peroxisome proliferative activated receptor, delta, nuclear hormone receptor 1, peroxisome proliferative activated receptor delta, OTTHUMP00000016256, OTTHUMP00000016257

Poids moléculaire: 49.9kDa

ID gène: 5467

NCBI Accession: NP_006229

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation