Acetylcholinesterase anticorps (C-Term)

N° du produit ABIN185357

L’anticorps Chèvre Polyclonal anti-Acetylcholinesterase a été validé pour WB, ELISA, IF et FACS. Il convient pour détecter Acetylcholinesterase dans des échantillons de Humain.

Aperçu rapide pour Acetylcholinesterase anticorps (C-Term) (ABIN185357)

Antigène: Acetylcholinesterase (AChE)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Acetylcholinesterase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-Acetylcholinesterase anticorps

Épitope: C-Term

Fonction: ACHE

Séquence: QFDHYSKQDR CSDL

Specificité: This antibody is expected to recognise isoform NP_000656.1 only (the ubiquitously expressed, hydrophillic form).

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence QFDHYSKQDRCSDL, from the C Terminus of the protein sequence according to NP_000656.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 70 kDa band observed in HepG2 and Jurkat cell lysates (calculated MW of 67.8 kDa according to NP_000656.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm, plasma membrane and nucleus of U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomm

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Acetylcholinesterase

Antigène: Acetylcholinesterase (AChE)

Autre désignation: ACHE

Sujet: ACHE, YT, acetylcholinesterase (YT blood group), ARACHE, N-ACHE, OTTHUMP00000211347, OTTHUMP00000211349, OTTHUMP00000211356, acetylcholinesterase, apoptosis-related acetylcholinesterase

ID gène: 43

NCBI Accession: NP_000656, NP_001269378

Pathways: Skeletal Muscle Fiber Development