PAI1 anticorps (Internal Region)

N° du produit ABIN185384

L’anticorps Chèvre Polyclonal anti-PAI1 a été validé pour WB, IHC et ELISA. Il convient pour détecter PAI1 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour PAI1 anticorps (Internal Region) (ABIN185384)

Antigène: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PAI1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-PAI1 anticorps

Épitope: Internal Region

Fonction: PAI1 / SERPINE1

Séquence: GFKIDDKGMA PALRH

Réactivité croisée: Chien, Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1, NP_001158885.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration: 4-6 μg/mL. A customer successfully used this product on Human Breast cancer.

Western Blot: Approx 48 kDa band observed in Human Liver and HeLa lysates, while approx. 45 kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1 kDa according to NP_000593.1). This molecular weight is routinely observed by other source

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-PAI1 (ABIN185384)

Kozaczuk, Selmi, Bednarek: "Bacterial expression, purification and angiogenesis-promoting activity of human thymosin ?4." dans: Protein expression and purification, Vol. 90, Issue 2, pp. 142-52, (2013) (PubMed).

Détails sur PAI1

Antigène: PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))

Autre désignation: SERPINE1

Sujet: SERPINE1, PAI1, PAI, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, serpin peptidase inhibitor, clade E (nexin, plasminogen activator in

Poids moléculaire: 45.1kDa

ID gène: 5054

NCBI Accession: NP_000593

Pathways: Signalisation p53, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration