CBX3 anticorps (Internal Region)

N° du produit ABIN185493

Cet anticorps anti-CBX3 est un anticorps Chèvre Polyclonal détectant CBX3 dans WB, IHC, ELISA, IF et FACS. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour CBX3 anticorps (Internal Region) (ABIN185493)

Antigène: CBX3 (Chromobox Homolog 3 (CBX3))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CBX3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-CBX3 anticorps

Épitope: Internal Region

Fonction: CBX3 / HP1 Gamma

Séquence: EAFLNSQKAG KEKD

Specificité: No cross-reactivity expected with HP1-alpha and HP1-beta. Reported variants represent identical protein: NP_009207.2, NP_057671.2

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-EAFLNSQKAGKEKD, from the internal region of the protein sequence according to NP_009207.2, NP_057671.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration: 2-3 μg/mL.

Western Blot: Approx. 20 kDa band observed in lysates of cell lines A431, HeLa, HepG2, NIH3T3 and NSO (calculated MW of 20.8 kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-CBX3 (ABIN185493)

Di Cello, Shin, Harbom, Brayton: "Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice." dans: Biochemical and biophysical research communications, Vol. 434, Issue 1, pp. 70-4, (2013) (PubMed).

Détails sur CBX3

Antigène: CBX3 (Chromobox Homolog 3 (CBX3))

Autre désignation: CBX3

Sujet: CBX3, HP1-GAMMA, chromobox homolog 3 (HP1 gamma homolog, Drosophila) , HGNC:1553, HECH, HP1Hs-gamma , HP1 gamma homolog, chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), heterochromatin protein HP1 gamma, heterochromatin-like protein 1

ID gène: 11335, 12417, 297093

NCBI Accession: NP_009207